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(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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Cell Signaling Technology Inc anti h3k4me2
(A and B) The relative occupancy <t>of</t> <t>H3,</t> H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using <t>ChIP-PCR</t> in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.
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Image Search Results


(A and B) The relative occupancy of H3, H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.

Journal: Oncotarget

Article Title: Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2

doi: 10.18632/oncotarget.19594

Figure Lengend Snippet: (A and B) The relative occupancy of H3, H3K4me1, H3K4me2, and H3K4me3 within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (A) or SENP2 (B). The graph shows the average of three independent experiments; mean ± S.D. **, p < 0.01. (C and D) The relative occupancy of CENP-A within α-satellite of chromosome 1 was analyzed using ChIP-PCR in cells stably expressing PIAS4 shRNA (C) or SENP2 (D). The data represent the means and SEM of three independent experiments.

Article Snippet: Chromatin-IP was performed using the ChIP Assay Kit (Millipore) according to the manufacturer’s protocol by using anti-H3 (cat. #14269), anti-CENP-A (cat. #2186), anti-H3K4me1 (cat. #5326), anti-H3K4me2 (cat. #9725), and anti-H3K4me3 (cat. #8580).

Techniques: Stable Transfection, Expressing, shRNA